Effects of Discontinuous Percoll Gradient Containing Alpha-linolenic Acid on Characteristics of Frozen-thawed Boar Spermatozoa
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چکیده
منابع مشابه
Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa.
The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-...
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Ovulation time of gilts was controlled by oral treatment with altrenogest (17-allyl-hydroxyestra-4,9,11-trien-3-one) followed by gonadotropic hormone injections. Gilts were laparotomized 42 to 44 h after injection of human chorionic gonadotropin. Spermatozoa were surgically placed into ligated oviducts of gilts after one of the following semen treatments: frozen and thawed in Beltsville thawing...
متن کاملComparison of CapriPure and Percoll density gradients for sperm separation of frozen- thawed goat spermatozoa
Frozen-thawed semen from six Boer bucks was pooled and used to compare the recently available commercial product CapriPure with Percoll for sperm quality. Sperm quality parameters such as sperm motility, concentration, membrane integrity (PI/CFDA), acrosome integrity (FITC-PNA) and mitochondrial activity (JC-1) were evaluated before and after sperm processing using CapriPure and Percoll density...
متن کاملComparative Study of Different Methods for Plasma Membrane Integrity Assessment of Frozen-thawed Boar Spermatozoa
In this study, different assays were used to assess the structural and functional integrity of the sperm plasma membrane following freezing-thawing of the whole ejaculates (WEs) and sperm-rich fractions (SRFs) of boar semen. Besides sperm viability assessments (motility and mitochondrial membrane potential using a mitochondrial specific dye, JC-1 with propidium iodide, PI), sperm plasma membran...
متن کاملFrozen boar spermatozoa: methods of thawing pellets.
In four experiments, the effects of pre-thaw delay, method of thawing, temperature of thawing solution and pellet volume on acrosome morphology and moti l i ty of" boar spermatozoa frozen by the pellet method were examined. Holding the frozen pellets for 0, 1.5, 3 or 4.5 min in a Styrofoam container before transferring them into the thawing solution resulted in maximum post-thaw percentages of ...
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ژورنال
عنوان ژورنال: Journal of Animal Reproduction and Biotechnology
سال: 2020
ISSN: 2671-4639,2671-4663
DOI: 10.12750/jarb.35.1.58